19 fungus were isolated from two different soil environments and a comparative analysis of their heavy metal tolerance was conducted. Among them, 18 fungus, i.e. Aspergillus sp., Trichoderma sp., Penicillium sp., Fusarium sp., were isolated respectively from the contaminated soil of Shuikoushan nonferrous metals mine in Hunan Province; 2 fungus, identified as Aspergillus niger., were isolated from non-contaminated soil in green land located in Langfang Economic Development Zone. Fungus isolated from the contaminated soil and noncontaminated green land as well as those reported previously are compared in terms of their heavy metal resistance characterized with the minimal inhibitory concentrations (MICs). As results, mechanism of fungal resistance of high concentrations of heavy metals was revealed. The fungus of heavy metal resistance primarily relies on the sites of their isolation, metal species, fungi species and metal speciation. Considering their high heavy-metal tolerance, e.g. MICs 144 mmol/L for Pb(Ⅱ), 36 mmol/L for Cd(Ⅱ) and Cu(Ⅱ), and 72 mmol/L for As(Ⅲ), the isolated fungus perform excellent and thus have higher potential for soil restoration.